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RNAi and plant gene function analysis methods and protocols by Hiroaki Kodama

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Published by Springer in New York .
Written in English


  • Plants,
  • Small interfering RNA,
  • Plant genetics,
  • Genetics,
  • Laboratory Manuals,
  • RNA Interference,
  • Laboratory manuals

Book details:

Edition Notes

Includes bibliographical references and index.

Statementedited by Hiroaki Kodama, Atsushi Komamine
SeriesSpringer protocols, Methods in molecular biology -- v. 744, Springer protocols, Methods in molecular biology (Clifton, N.J.) -- v. 744.
LC ClassificationsQK981 .R63 2011
The Physical Object
Paginationx, 244 p. :
Number of Pages244
ID Numbers
Open LibraryOL25050804M
ISBN 101617791229, 1617791237
ISBN 109781617791222, 9781617791239
LC Control Number2011926633

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: RNAi and Plant Gene Function Analysis: Methods and Protocols (Methods in Molecular Biology, Vol. ) (): Hiroaki Kodama, Atsushi Komamine: Books. Buy RNAi and Plant Gene Function Analysis (): Methods and Protocols: NHBS - Edited By: Hiroaki Kodama and Atsushi Komamine, Humana Press.   Double-stranded (ds)RNA interference (RNAi) is widely used for functional analysis of plant genes and is achieved via generating stable transformants expressing dsRNA in planta. This study demonstrated that RNAi can also be utilized to examine gene functions in protoplasts. Because protoplasts are nongrowing cells, effective RNAi-triggered gene silencing .   Abstract. Controlling gene expression during plant development is an efficient method to explore gene function and RNA interference (RNAi) is now considered as a powerful technology for gene functional by: 3.

Overview of plant RNAi / Yuichiro Watanabe --Caveat of RNAi in plants: the off-target effect / Muthappa Senthil-Kumar and Kirankumar S. Mysore --Plant gateway vectors for RNAi as a tool for functional genomic studies / Toshiya Muranaka --Heat-inducible RNAi for gene functional analysis in plants / Frederic Masclaux and Jean-Philippe Galaud. Get this from a library! RNAi and plant gene function analysis: methods and protocols. [Hiroaki Kodama; Atsushi Komamine;] -- The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative. Additional info for RNAi and Plant Gene Function Analysis: Methods and Protocols. Example text. Fragments can be stored at –20 C for later use. 6. Ligate 30–75 ng of open vector pBSint11 with approximately threefold molar excess of the sense fragment with T4 DNA ligase in 1× ligation buffer and a final volume of 20 μL overnight at 15 C. /5(41).   Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gain- or loss-of-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functional studies in hexaploid wheat (Triticum .

  Therefore, RNAi in protoplasts described here illustrated a rapid, affordable, and potent reverse-genetic approach that is complementary to existing genetic tools, as it allows expediting functional analysis of plant genes and, as detailed in “Conclusion and Future Perspectives,” is particularly applicable for biochemical studies for Cited by: We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med –, ). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in rapid, efficient, and stable transduction into mouse skin. Our technique greatly extends the available molecular Cited by: The discovery of RNA interference (RNAi) and its utilization in downregulation of specific target transcripts have revolutionized gene function analysis and elucidation of many key biochemical/genetic pathways. The insights into gene function, combined with a technology that made silencing of gene function possible using the potent, highly specific and selective RNAi Cited by: 1.   Abstract. RNAi refers to several different types of gene silencing mediated by small, dsRNA molecules. Over the course of 20 years, the scientific understanding of RNAi has developed from the initial observation of unexpected expression patterns to a sophisticated understanding of a multi-faceted, evolutionarily conserved network of mechanisms that Cited by: